Efficient and consistent dissociation of adherent cell cultures using TrypsiNNex®
Adherent cell culture systems are vital in biomanufacturing, serving as a foundation for development and production of various vaccines and therapeutics. As a key cell dissociation reagent that enables culture expansion, trypsin can impact the efficiency and scalability of a production process. Our recombinant enzyme TrypsiNNex® was designed as a high-purity, animal component-free cGMP trypsin that […]
Cell culture-based production of biologically active influenza viruses using TrypsiNNex®
Cell culture-based production of influenza virus for vaccine manufacturing is faster and easier scalable than egg-based methods. In addition to being more stable, virus production in cell lines also gives higher yield and is safer to the end user. Influenza vaccines produced in mammalian cell lines require activation by trypsin. Trypsin is used to cleave […]
Reliable MSC expansion and differentiation using animal-free cell culture conditions
Enabling reliable MSC expansion under animal-free conditions Translating robust, research-grade MSC cultures into defined, GMP-ready processes can be challenging, especially when replacing serum and animal-derived reagents with animal-origin–free alternatives. Changes in culture components can affect growth, viability, and phenotype, often requiring additional optimization and validation. In this application note, we demonstrate how GMP-grade TrypsiNNex® and […]
