The challenge of maintaining iPSC cultures
The rapid advancement in the fields of regenerative medicine and drug discovery has been facilitated by the use of induced pluripotent stem cells (iPSCs). However, achieving efficient and stable cultures that maintain pluripotency while promoting optimal growth and proliferation is a key challenge.
Studies have highlighted the importance of optimizing individual components in iPSC culture media. Insulin is one such reagent that has been shown to enhance overall health of iPSC cultures. Recombinant Insulin is a reagent that specifically meets the demanding and stringent requirements of human iPSC culture, maintaining high quality standards.
Benefits of using Recombinant Insulin in iPSC cultures
Our application note demonstrates that incorporating recombinant insulin into iPSC culture media significantly enhanced cell growth and pluripotency. After testing various insulin concentrations, we identified an optimal concentration that was subsequently used in further assessments. The use of insulin in iPSC culture also enhanced cell attachment in single-cell and aggregate seeding, therefore increasing the likelihood of successful downstream assays. Importantly, long-term maintenance of iPSC cultures in the presence of insulin did not dampen pluripotency due to spontaneous differentiation.
Our well-characterized insulin can boost the quality of your cultures, reduce variability, and enhance the reliability of iPSC production and subsequent findings. These improvements can streamline experimental processes, ultimately reducing production time and costs. Successful iPSC cultures are highly dependent on precise and optimized media composition. Choosing our insulin reagent at the outset of iPSC-based applications can help avoid barriers to scaling up production at a later stage. By selecting reagents that meet stringent regulatory standards, you can ensure a smooth transition to therapeutic development.
By integrating our Recombinant Insulin into your iPSC culture procedures, you can expect:
- Increased proliferation and attachment of iPSCs that are crucial for large-scale applications.
- Long-term stability without compromising consistency and quality.
- Optimized culture conditions that are ideal for consistent and reproducible experiments.
Download the application note
Ready to optimize and enhance your iPSC culture conditions? Download our detailed application note to learn more about the study. Discover the specific benefits and optimal concentrations of our insulin that will enhance growth, stability, and reproducibility of your iPSC cultures.
