Optimization of CHO-S cell culture medium by supplementation with non-animal derived components using Design of Experiments (DoE)

CHO cells are one of the most widely used platforms for the production of biopharmaceuticals. Increased demand for safety and reliability has moved the standard for CHO cell culture media from Serum to Serum free and further on to chemically defined media.

UAB in collaboration with Novo Nordisk Pharmatech (world’s largest supplier of recombinant insulin) has shown that addition of animal origin free insulin to three leading commercially available off-the-shelf chemically defined media under batch-mode culture resulted in significant increases in viable cell density, leading up to 53 % cell growth improvement and 66% of targeted protein production.

In addition to this benefit insulin has been proven to aid in the expression of difficult to express proteins.

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Chantale Julien
Chantale Julien
Product Manager

Phone: +45 4029 7906