Are you looking for a method to increase the expression of recombinant proteins in Chinese Hamster Ovary (CHO) cells? Then, this case study is for you.
The CHO cell line is widely used to produce recombinant proteins due to its high growth capacity and productivity. Adapting cell culture media for each application is critical to exploiting these features for high-performance and cost-effective process development.
In this study, we show the effects of animal-free recombinant insulin supplementation on CHO-K1 cell proliferation and productivity of IgG protein in fed-batch culture.
The results show that supplementing recombinant insulin to the basal media significantly improves cell proliferation and IgG productivity (up to 6g/L).
Find the case study here to learn more about the experiment.