You can increase viable CHO cell density by supplementing with recombinant Insulin Human AF. CHO cells are one of the most widely used platforms to produce biopharmaceuticals.
Increased demand for safety and reliability has moved the standard for CHO cell culture media from Serum to Serum free, and further on to chemically defined media.
UAB (Universitat Autònoma de Barcelona), in collaboration with Novo Nordisk Pharmatech A/S, has shown that the addition of animal origin free insulin to three leading commercially available, off-the-shelf chemically defined media, resulted in significant increases in viable cell density under batch-mode culture. This led to improve cell growth up to 53 % and 66% of targeted protein production.
In addition to this benefit, insulin has been proven to aid in the expression of proteins that are difficult to express.
You can read or download the result of the study here.