Effect of Insulin on influence production in HEK293 Cells
This article explores the effect of adding insulin to increase influenza production. Mammalian cells are considered an alternative to eggs for the production of influenza vaccines. HEK293SF-3F6 is a suspension GMP cell line that grows in serum-free media, and influenza production has been achieved in flasks and large bioreactors. In order to boost influenza production, insulin was added to the cultures. This growth factor was chosen because it has cell survival effects, it acts on cellular signaling pathways that are exploited by the influenza virus and it is approved by regulatory agencies. Depending on the media the effective insulin concentration varied.
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