Application for recombinant Insulin Human AF
- Application for recombinant Insulin Human AF
- Cookies
- Front Page
- Insights
- Onboarding of new employees
- Quats applications
- Registrant group for Cetrimonium Bromide (CTAB)
- Registrant group for Reaction mass of C12 trimethylammonium bromide and C14 trimethylammonium bromide
- Registrant group for Tetradonium Bromide
- SDS
- Products
- Applications for Quats
- Video
- Why us
- Download & Publications
- Company
- Contact us
HEK293
Increase specific influenza production with recombinant insulin
Mammalian cells are considered an alternative to eggs for the production of influenza vaccines. HEK293SF-3F6 is a suspension GMP cell line that grows in serum-free media, and influenza production has been achieved in flasks and large bioreactors.
National Research Council Canada (NRC) in collaboration with Novo Nordisk Pharmatech (world’s largest supplier of recombinant insulin) has shown that addition of animal origin free insulin to commercial available media and in-house proprietary media in HEK 293SF-3F6 batch-mode culture, resulted in significant increases in cell growth, leading up to 4-fold increase compared to insulin free media formulations.
In addition to cell growth, the study aimed to address the effect of Insulin on HEK-based Influenza Production, since the effect on Influenza virus productivity was not known.
Insulin has been proved already to positively affect cell survival, by promoting anti-apoptotic and mitogenic pathways, to interact on cellular signalling pathways that are exploited by the influenza virus and it has the benefit of being already approved by regulatory agencies.
This study has shown that addition of animal origin free insulin to commercial available media and in-house proprietary media in HEK 293SF-3F6 micro-bioreactor culture, resulted in significant increase in H1N1 and H3N2 Influenza subtypes , leading to up to 1,9-fold increase compared to insulin free media formulations.
You can read or download the poster here.
In case of questions to the text above, please contact us.
Customer service
Insulin sales
Quats sales
Distributors
Asia Pacific
DKSH Holding Ltg.
Wiesenstrasse 8,
P.O. Box. 888
8034 Zürich
Switzerland
Phone: +41 44 386 7272
Fax: +41 44 386 7282
Austria
Selectchemie AG Schweiz
Etzelstrasse 42
P.O. Box 772
CH-8038 Zürich
Tel. +41 44 487 96 11
Fax. +41 44 487 96 90
Bangladesh
Signet
A-801, Crescenzo
C/38-39, G-Block
Bandra Kurla Complex
Mumbai - 400 051
India
Tel: +91 22 61462725
Fax: +91 22 61462726
Brazil
Eskisa S.A
Rua Guilherme Barbosa de Melo, 83 / 87
Brooklin Novo
CEP 04571-160 - São Paulo – SP
Brazil
Tel.: (11) 5536-0533 / 2385-0500
Fax.: (11) 5536-4152
Canada
A&C
3010 de Baene
Saint-Laurent, Quebec
H4S 1L2
Canada
Tel: +1 514 336 1493
Toll Free: +1 800 361 9234
Fax: +1 514 336 1768
Eastern Europe
Selectchemie AG Schweiz
Etzelstrasse 42
P.O. Box 772
CH-8038 Zürich
Tel. +41 44 487 96 11
Fax. +41 44 487 96 90
France
Quimdis
71 RUE ANATOLE FRANCE
92300 LEVALLOIS PERRET
FRANCE
Tel + 33 (0) 1 55 46 50 00
Fax + 33 (0) 1 47 39 91 90
Germany
Azelis Germany
Weeserweg 23
47804 Krefeld
Tel: +49 (0) 2151 7286 90
Fax. +49 (0) 2151 7286 99
India
Signet
A-801, Crescenzo
C/38-39, G-Block
Bandra Kurla Complex
Mumbai - 400 051
India
Tel: +91 22 61462725
Fax: +91 22 61462726
Italy
IMCD Italia SpA.
Via XXV Aprile, 4
San Donato, MI 20097
Phone : +39 02 557 091
Fax : +39 02 557 09 210
Portugal
Azelis Portugal
World Trade Center Almeda Park
Plaça de la Pau, s/n. Edificio 8, 1ª planta
Cornellà de Llobregat (Barcelona)
Spain - 8940
+34 93 409 90 70
+34 93 339 21 62
South Africa
Warren Chem Ltd.
11 Mansell Road Killarney Gardens Cape Town,
Western Cape 7441
Tel: +27 21 556 1920
Fax: +27 21 556 1905
Spain
Azelis Spain
World Trade Center Almeda Park
Plaça de la Pau, s/n. Edificio 8, 1ª planta
8940 Cornellà de Llobregat (Barcelona)
Tel: +34 93 409 90 70
Fax +34 93 339 21 62
Switzerland
Selectchemie AG Schweiz
Etzelstrasse 42
P.O. Box 772
CH-8038 Zürich
Tel. +41 44 487 96 11
Fax. +41 44 487 96 90
Turkey
Kura Ltd.
Kore Sehitleri Cad.Yzb.Kaya Aldogan Sk. Nr:12/3
34394 Zincirlikuyu - Istanbul
Phone: +90 212 347 25 30
Fax: +90 212 347 35 30
UK & Ireland
Azelis UK & Ireland
Foxholes Business Park
John Tate Road
Hertford
Herts
SG13 7YH
United Kingdom
Tel:+44 (0) 1992 825555
Fax +44 (0) 1992 825566
USA
Brenntag Specialties
100 Coolidge Street
South Plainfield, NJ 07080
Domestic
Phone: (800) 732-0562
Fax: (800) 543-1484
International
Phone: (908) 561-6100
Fax: (908) 757-3488
